Thermo Fisher Scientific Human CDK4 Recombinant Protein
정제된 인간 CDK4 재조합 단백질로, N-말단 His-tag를 포함합니다. Western blot 등 다양한 면역학적 분석에서 양성 대조군으로 사용 가능합니다. E. coli 발현, >95% 순도, 액상 형태로 제공되며 -20°C에서 보관합니다.
- 카탈로그번호
- RP7553x (3개 옵션)
- 카테고리
- Protein
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Human CDK4 Recombinant Protein
Applications
- Western Blot (WB): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Amino acids Met1–Glu303 containing an N-terminal His-tag |
| Tag | His-tag |
| Molecular Weight | 38 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95%, by SDS-PAGE under reducing conditions |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.081 mg/mL |
| Purification | Purified |
| Storage Buffer | 0.01M Bis-tris propane, pH 9.0, with 0.1M NaCl, 50% glycerol |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
This product contains purified recombinant human CDK4 protein with an N-terminal hexahistidine tag.
It may be used as a positive control in various immunological assays.
Target Information
CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and plays a crucial role in controlling cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). It mediates phosphorylation of the C-terminal region of the Rb protein, leading to transcriptional repression of the E2F complex.
CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of G1 cyclin-dependent kinases CDK4 and CDK2.
Although CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein, they exhibit different residue selectivity in vitro: CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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