Thermo Fisher Scientific Human CDK4 Recombinant Protein

Applications

• Western Blot (WB): Assay-dependent
• Control (Ctrl): Assay-dependent

Product Specifications

Product Specific Information

This product contains purified recombinant human CDK4 protein with an N-terminal hexahistidine tag.
It may be used as a positive control in various immunological assays.

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and plays a crucial role in controlling cell proliferation during the G1 phase of the cell cycle.
CDK4 forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). It mediates phosphorylation of the C-terminal region of the Rb protein, leading to transcriptional repression of the E2F complex.
CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of G1 cyclin-dependent kinases CDK4 and CDK2.
Although CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein, they exhibit different residue selectivity in vitro: CDK6 phosphorylates Thr821, while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.