Thermo Fisher Scientific Human E-selectin (CD62E) Recombinant Protein
Recombinant human E-selectin (CD62E) 단백질로, 면역학적 분석의 양성 대조군으로 사용 가능. E. coli 발현 시스템에서 생산되며 N-말단 His-tag 포함. 95% 이상 순도로 정제되어 있으며, 염증 반응 연구에 적합. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
Control (Ctrl)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | E. coli |
| Amino Acid Sequence | Amino acids Asn179–Leu557 containing an N-terminal His-tag |
| Tag | His-tag |
| Class | Recombinant |
| Type | Protein |
| Purity | >95%, by SDS-PAGE under reducing conditions |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.11 mg/mL |
| Purification | Purified |
| Storage Buffer | 20 mM Tris HCl, pH 8, with 50% glycerol, 100 mM NaCl, 1 mM EDTA |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
Product Specific Information
RP-75539 contains purified recombinant human E-selectin protein with an N-terminal hexahistidine tag.
RP-75539 may be used as a positive control in many immunological assays.
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion also occurs in capillaries and small venules at inflammation sites. This process involves a cascade of adhesion receptors, including members of the selectin and immunoglobulin superfamilies.
E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation by proinflammatory cytokines. In vitro studies show IL-1, TNFα, and bacterial lipopolysaccharides induce E-selectin transcription via an NFκB-dependent signaling cascade. E-selectin is associated with endothelial cells in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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