Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Rabbit polyclonal antibody targeting human Histone H2A.X for WB and IHC applications. Detects DNA damage marker γ-H2AX phosphorylation. Supplied as liquid, unconjugated form, 0.16 mg/mL concentration. Recommended storage at -20°C, avoid freeze/thaw cyc...
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- PA5120125
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–143 of human Histone H2AX (NP_002096.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.16 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913697 |
Product Specific Information
Positive test controls include: K-562, HeLa, Mouse testis, Rat testis.
The target is usually found in the following locations: Chromosome, Nucleus.
Immunogen sequence:
MSGRGKTGGK ARAKAKSRSS RAGLQFPVGR VHRLLRKGHY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGG VTIAQGGVLP NIQAVLLPKK TSATVGPKAP SGGKKATQAS QEY
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome. In eukaryotes, DNA double-strand breaks trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, producing γ-H2AX. This phosphorylation can be detected by Western blot or immunofluorescence to assess DNA damage frequency.
Phosphatidylinositol 3-kinases are implicated in H2AX phosphorylation, though it remains unclear if ATM is the primary kinase or if DNA-PK and ATR also contribute. Structurally, H2A.X contains 143 amino acid residues and is synthesized during G1 and S-phase. Its mRNA variants differ in polyA tail presence, with one form behaving as replication-type and the other as basal-type histone mRNA. Histone H2A.X maps to human chromosome 11q23.2–q23.3 and plays a role in chromatin structure and DNA repair through interaction with MDC1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(이미지 파일명: PA5-120125_Histone_H2A.X_P16104-1_Rabbit.svg, PA5-120125_Histone_H2A.X_P16104-1_Rabbit_PDP.jpeg)
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