Thermo Fisher Scientific PDGF-BB Polyclonal Antibody, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human PDGF-BB if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PDGF-BB,uniProtId:P01127-1,ncbiNodeId:9606,antigenRange:82-190,antigenLength:241,antigenImageFileName:500-P47-100UG_PDGF-BB_P01127-1_Rabbit.svg,antigenImageFileNamePDP:500-P47-100UG_PDGF-BB_P01127-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930563

Product Specific Information

AA Sequence of recombinant protein: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB. Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography employing an immobilized Human PDGF-BB matrix.

Sandwich ELISA: To detect Human PDGF-BB by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PDGF-BB (500-P47Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PDGF-BB.

Western Blot: To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-BB is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.