Thermo Fisher Scientific PDGF-BB Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-BB. Anti-Human PDGF-BB-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hPDGF-BB by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PDGF-BB (500-P47) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hPDGF-BB.

Western Blot:
To detect hPDGF-BB by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL.
With compatible secondary reagents, the detection limit for Recombinant hPDGF-BB is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P47BT-1MG is provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a member of the platelet-derived growth factor family. Members of this family are mitogenic factors for cells of mesenchymal origin and characterized by a motif of eight cysteines.
This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with PDGF-α (PDGF-AB), connected by disulfide bonds.
Mutations are associated with meningioma and dermatofibrosarcoma protuberans due to unregulated growth factor expression.
Two alternatively spliced transcript variants encoding different isoforms have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.