Thermo Fisher Scientific PDGF-AA Polyclonal Antibody, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-AA. Anti-Human PDGF-AA-specific antibody was purified by affinity chromatography employing an immobilized Human PDGF-AA matrix.

Sandwich ELISA:
To detect Human PDGF-AA by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PDGF-AA (500-P46Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human PDGF-AA.

Western Blot:
To detect hPDGF-AA by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant hPDGF-AA is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It is secreted from the alpha-granules of platelets activated during blood coagulation. PDGF consists of dimeric isoforms (PDGF-AA, AB, BB) that are differentially expressed in various cell types and act through two receptors (alpha and beta). PDGF isoforms are potent mitogens for connective tissue cells and are chemotactic for fibroblasts, smooth muscle cells, and immune cells. It also plays roles in collagen synthesis, cytokine modulation, and neuronal survival and regeneration in the CNS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.