Thermo Fisher Scientific TL1A Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific TL1A Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
QLRAQGEASV QFQALKGQEF APSHQQVYAP LRADGDKPRA HLTVVRQTPT QHFKNQFPAL HWEHELGLAF TKNRMNYTNK FLLIPESGDY FIYSQVTFRG MTSECSEIRQ AGRPNKPDSI TVVITKVTDS YPEPTQLLMG TKSVCEVGSN WFQPIYLGAM FSLQEGDKLM VNVSDISLVD YTKEDKTFFG AFLL

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TL-1A. Anti-Human TL-1A-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hTL-1A by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TL-1A (500-P240) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hTL-1A.

Western Blot:
To detect hTL-1A by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant hTL-1A is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P240BT-1MG will be provided as 2 × 500 µg.

Target Information

The protein encoded by this gene is a cytokine belonging to the tumor necrosis factor (TNF) ligand family. It is abundantly expressed in endothelial cells but not in B or T cells. Expression is inducible by TNF and IL-1 alpha. This cytokine binds to receptors TNFRSF25 and TNFRSF21/DR6, activates NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. It also inhibits endothelial cell proliferation, functioning as an angiogenesis inhibitor. An additional isoform from an alternatively spliced transcript has been reported but its sequence is undetermined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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