Thermo Fisher Scientific Adiponectin Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 Rabbit Polyclonal Anti-Human Adiponectin 항체로, Western Blot 및 Sandwich ELISA에 최적화되어 있습니다. 고순도 재조합 단백질로 면역하여 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 인슐린 저항성 및 지방 대사 연구에 적합합니다.
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Thermo Fisher Scientific Adiponectin Polyclonal Antibody, Biotin, PeproTech
Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Hi-5 Insect cells-derived Recombinant Human Adiponectin |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929988 |
Product Specific Information
AA Sequence of recombinant protein:
RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHITVYM KDVKVSLFKK DKAMLFTYDQ YQENNVDQAS GSVLLHLEVG DQVWLQVYGE GERNGLYADN DNDSTFTGFL LYHDTN.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Adiponectin. Anti-Human Adiponectin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human Adiponectin by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Adiponectin (500-P239) as a capture antibody, allows detection of at least 2000–4000 pg/mL of Recombinant Human Adiponectin.
Western Blot:
For Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, detection limit is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Packaging:
500-P239BT-1MG is provided as 2 × 500 µg.
Target Information
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates lipid and glucose metabolism. It plays roles in homeostasis and obesity. Decreased adiponectin levels are associated with obesity, insulin resistance, and type 2 diabetes. In mice, adiponectin improves insulin signaling and reduces intramyocellular lipid levels via activation of AMP kinase and PPAR-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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