Thermo Fisher Scientific HSP27 Monoclonal Antibody (5D12-A12), PerCP
HSP27 단백질을 인식하는 PerCP 결합 단클론 항체로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합. 사람 시료 반응성, 단클론(Mouse IgG2b) 형식. 27kDa 단백질 검출에 특이적이며 Alpha B crystallin과 교차반응 없음. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000 | |
| Immunohistochemistry (IHC) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ELISA | Assay-dependent | |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D12-A12 |
| Immunogen | Full length human HSP27 (UniProt ID: P04792-1, antigen range: 1–205) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931661 |
Available Formats
Product Specific Information
- 0.5 µg/mL of MA5-45207 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot using Goat anti-mouse IgG:HRP as the secondary antibody.
- Detects approximately 27 kDa.
- No cross-reactivity to Alpha B crystallin.
- Very limited cross-reactivity to other species.
Target Information
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein acting as a molecular chaperone that maintains denatured proteins in a folding-competent state. It plays roles in stress resistance and actin organization. Through its chaperone activity, HSP27 regulates biological processes including phosphorylation and axonal transport of neurofilament proteins.
Mutations in the HSPB1 gene can cause Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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