Thermo Fisher Scientific HSP70 Monoclonal Antibody (5A5), FITC
HSP70 단백질을 인식하는 FITC 결합 단일클론 항체로, 다양한 종에 반응합니다. Western blot, IHC, ICC, IP 등 다중 응용에 적합하며, 단백질 G로 정제된 고순도 제품입니다. 4°C 암소 보관, 형광 분석용으로 최적화되었습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Immunoprecipitation (IP) | 1 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Chicken, Fruit fly, Fish, Human, Mouse, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A5 |
| Immunogen | Human recombinant HSP70 overexpressed in E. coli |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.2), 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931663 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45209 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 70 kDa. May detect HSP70, HSC70, Grp78, and HSP72.
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including elevated temperatures. The HSP70 family includes several conserved proteins such as HSP70, HSP72, HSC70, GRP78, and HSP75, which are involved in protein folding, stabilization, and cellular stress response.
- HSP70: Strongly inducible in all organisms, constitutively expressed in primate cells
- HSP72: Induced exclusively under stress conditions
- HSC70: Constitutively expressed, involved in uncoating clathrin-coated vesicles
- GRP78 (BiP): ER-localized glucose-regulated protein
- HSP75 (p75): Mitochondrial protein
HSP70 stabilizes existing proteins against aggregation, assists in folding of newly synthesized proteins, and participates in the ubiquitin-proteasome pathway. The HSP70 gene is located in the MHC class III region along with related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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