Thermo Fisher Scientific HSP27 Monoclonal Antibody (5D12-A12), FITC
HSP27 단백질을 검출하기 위한 FITC 결합 마우스 단일클론 항체. Western blot, IHC, ICC 등 다양한 응용 가능. 인간 시료 반응성, 단백질 G 정제, 4°C 암소 보관. 교차 반응성이 낮고 높은 특이성 제공.
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Thermo Fisher Scientific HSP27 Monoclonal Antibody (5D12-A12), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5D12-A12 |
| Immunogen | Full length human HSP27 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.2, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931660 |
Additional Formats
Product Specific Information
0.5 µg/mL of MA5-45206 was sufficient for detection of HSP27 in 10 µg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 27 kDa.
No cross-reactivity to Alpha B crystallin.
Very limited cross-reactivity to other species.
Target Information
HSPB1 (heat shock protein beta-1 or HSP27) is a small heat shock protein that functions as a molecular chaperone, maintaining denatured proteins in a folding-competent state. It plays roles in stress resistance and actin organization.
Through its chaperone activity, HSP27 regulates biological processes such as phosphorylation and axonal transport of neurofilament proteins.
Mutations in this gene can result in Charcot-Marie-Tooth disease 2F and distal hereditary motor neuronopathy type 2B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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