Thermo Fisher Scientific HSP70 Monoclonal Antibody (5A5), APC
APC 형광으로 표지된 HSP70 단클론 항체(클론 5A5)로, Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합합니다. 인간 및 여러 종에 반응하며, 단백질 스트레스 반응 연구에 유용합니다. 단백질 G 정제, 액상 형태, 4°C 보관.
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Thermo Fisher Scientific HSP70 Monoclonal Antibody (5A5), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Immunoprecipitation (IP) | 1 µg |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Amphibian, Chicken, Fruit fly, Fish, Human, Mouse, Rat, Yeast |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5A5 |
| Immunogen | Human recombinant HSP70 overexpressed in E.coli |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.2, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931662 |
Additional Formats
- FITC: MA5-45209
- PerCP: MA5-45210
- Request custom conjugation available
Product Specific Information
1 µg/mL of MA5-45208 was sufficient for detection of HSP70 in 20 µg of heat-shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 70 kDa. May detect HSP70, HSC70, Grp78, and HSP72.
Target Information
HSP70 is a heat shock protein expressed in response to various biological stresses, including high temperatures. The HSP70 family includes HSP70, HSP72, HSC70, GRP78, and HSP75, all of which are highly conserved across organisms.
HSP70 stabilizes existing proteins against aggregation, assists in folding newly translated proteins, and participates in the ubiquitin-proteasome pathway. The HSP70 gene is located in the MHC class III region alongside related genes encoding similar proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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