Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (STA), Biotin, eBioscience

Applications

Product Specifications

Product Specific Information

The STA monoclonal antibody reacts with human CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to Integrin α4β1 (VLA-4, CD49d/CD29) and Integrin α4β7 (LPAM-1). Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role for this antigen in the inflammatory response.

Applications Reported: STA has been reported for use in flow cytometric analysis.

Applications Tested: The STA antibody has been tested by flow cytometric analysis of TNFα-activated HUVEC cells. This can be used at ≤0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may play a role in various inflammatory diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.