Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (429), Biotin, eBioscience
마우스 CD106(VCAM-1)을 인식하는 단클론 항체로, Biotin으로 표지되어 있습니다. Flow Cytometry에 최적화되어 있으며, 0.25 µg/test 권장. 염증 반응 및 세포 부착 연구에 활용 가능. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 6 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 16 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 429 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466559 |
| Filtration | 0.2 µm post-manufacturing filtered |
Additional Formats
- Unconjugated
- APC
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- Request custom conjugation
Product Specific Information
The 429 monoclonal antibody reacts with mouse CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and upregulate it upon cytokine stimulation. CD106 binds to integrin alpha4beta1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which play key roles in early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 suggests its role in inflammatory responses.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometry using mouse bone marrow cells at ≤0.25 µg/test. Optimal titration recommended for specific assays.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration and adhesion, participates in signal transduction, and is implicated in various inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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