Thermo Fisher Scientific Cystatin A Monoclonal Antibody (CSTA, 3553)
인간 Cystatin A 단백질을 인식하는 Mouse monoclonal antibody. Western blot, IHC(P), Peptide Array에 사용 가능. Protein A/G 정제 및 PBS 저장. 4°C 보관, 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CSTA, 3553 |
| Immunogen | Recombinant fragment of human CSTA protein (around aa 1–98) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some members are active cysteine protease inhibitors, while others have lost or never acquired this inhibitory activity.
There are three inhibitory families in the superfamily:
- Type 1 cystatins (stefins)
- Type 2 cystatins
- Kininogens
This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and cathepsins B, H, and L.
The protein serves as one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance.
Stefins have been proposed as prognostic and diagnostic tools for cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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