Thermo Fisher Scientific CX3CL1 (Fractalkine) Polyclonal Antibody, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

1:300-1:800

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468501

Product Specific Information

Description: The rabbit polyclonal antibody reacts with recombinant and natural mouse, rat, and human fractalkine (CX3CL1); the antibody was generated using E. coli-expressed chemotactic domain of human CX3CL1 as an immunogen. CX3CL1, also known as neurotactin or fractalkine, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor and consists of a chemokine domain of 76 amino acids, a mucin stalk of 241 amino acids, a putative transmembrane domain of 18 amino acids, and an intracellular tail of 37 amino acids. Within the chemokine domain, the first two cysteine residues are separated by 3 amino acids (CX3C). CX3CL1 message is found in high abundance in the brain, kidney, lung and heart tissues. CX3CL1 is chemotactic for monocytes and other leukocytes including NK cells and may play a role in brain inflammation.

Applications Reported: This polyclonal has been reported for use in immunoblotting (WB), immunoprecipitation, and immunohistochemistry. Cross-reactivity to CX3CL1 of other species has not been determined.

Applications Tested: To achieve best results, titrate antibody by dilution for each application as follows:

Western Blot 1:1000-1:5000

Immunoprecipitation 1:300-1:800

Immunohistochemistry 1:100-1:500.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.