Thermo Fisher Scientific Cystatin A Monoclonal Antibody (CSTA, 3553)
인간 Cystatin A 단백질을 인식하는 마우스 단클론 항체입니다. Western blot, IHC(파라핀 절편), Peptide Array 등에 사용 가능합니다. 단백질 A/G로 정제된 액상 형태이며, 보존제 없이 PBS(pH 7.4)에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 2–4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | - |
| Peptide Array (Array) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CSTA, 3553 |
| Immunogen | Recombinant fragment of human CSTA protein (around aa 1–98) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
The cystatin superfamily encompasses proteins containing multiple cystatin-like sequences. Some members act as active cysteine protease inhibitors, while others have lost or never acquired this activity.
There are three inhibitory families in the superfamily:
- Type 1 cystatins (stefins)
- Type 2 cystatins
- Kininogens
This gene encodes a stefin that functions as a cysteine protease inhibitor, forming tight complexes with papain and cathepsins B, H, and L.
The protein contributes to the formation of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance.
Stefins have been proposed as prognostic and diagnostic tools for cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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