Thermo Fisher Scientific CREB Recombinant Rabbit Monoclonal Antibody (3H8L15)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as an approximately 150 kDa band and, upon reduction, generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Improved specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to various extracellular signals such as neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors.

Activation of CREB depends on the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK, as well as p38 MAP kinase and MSK1. Although CREB binds DNA independent of its phosphorylation state, only the phosphorylated form is transcriptionally active.

CREB binding protein (CBP), a transcriptional coactivator, interacts with CREB at serine 133. CREB is involved in cellular processes including circadian rhythm synchronization, adipocyte differentiation, and learning and memory. In humans, the CREB gene is located on chromosome 2q.

Diseases associated with CREB dysfunction include Alzheimer’s disease, histiocytoma, and soft tissue melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.