Thermo Fisher Scientific IFN gamma Recombinant Rabbit Monoclonal Antibody (22H17L20)

Applications

• Western Blot (WB): 1–3 µg/mL

Product Specifications

Product Specific Information

Intact IgG appears on a non-reducing gel as a ~150 kDa band and upon reduction generates a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulation
• Broader immunoreactivity due to larger rabbit immune repertoire

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin, and other cytokines.

• IFN gamma is a dimeric glycoprotein consisting of two 146 amino acid subunits (~45 kDa homodimer).
• On SDS-PAGE, it appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
• Biological activity is highly species-specific; human IFN gamma does not cross-react with mouse.
• Functions include antiviral activity, tumor antiproliferative activity, induction of MHC I and II, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes.
• Activation occurs via IFN gamma receptor I and II, triggering the JAK-STAT pathway.
• Human IFN gamma shows ~40% sequence homology with mouse IFN gamma.
• Upregulated by IL2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
• Gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지