Thermo Fisher Scientific Tau Monoclonal Antibody (SP70)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
• Following antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
• Suggested positive control: breast adenocarcinoma.

Target Information

Tau is a neuronal microtubule-associated protein predominantly localized on axons. It promotes tubulin polymerization and stabilizes microtubules.

• The C-terminus binds axonal microtubules, while the N-terminus binds neural plasma membrane components, suggesting a linker function.
• Axonal polarity is influenced by TAU/MAPT localization in the neuronal cell body domain defined by the centrosome.
• Short isoforms contribute to cytoskeletal plasticity; longer isoforms stabilize microtubules.
• Hyper-phosphorylated Tau is the main component of paired helical filaments (PHF), forming neurofibrillary lesions in Alzheimer’s disease (AD).
• Hyper-phosphorylation impairs Tau’s microtubule-binding function, leading to microtubule destabilization and neuronal degeneration.
• Tau is phosphorylated by several kinases, including GSK-3β, PKA, cdk5, and casein kinase II.
• Hyper-phosphorylated Tau is also found in other CNS disorders such as Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.