Thermo Fisher Scientific Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (SP50)
Phospho-S6 (Ser235, Ser236) 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Monoclonal Antibody (SP50). Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용 가능. 고순도 Protein A/G 정제, 4°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:200 |
| Dot Blot (DB) | 0.123 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP50 |
| Immunogen | Synthetic peptide derived from the region of human S6 ribosomal protein containing phosphorylated serines 235 and 236 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.054 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537916 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is breast ductal carcinoma.
Target Information
Ribosomes catalyze protein synthesis and consist of a small 40S and a large 60S subunit, composed of 4 RNA species and approximately 80 distinct proteins.
This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit, belonging to the S6E family.
It is the major substrate of ribosomal protein kinases, with phosphorylation occurring on five C-terminal serine residues in response to stimuli such as growth factors, tumor promoters, and mitogens.
Dephosphorylation occurs during growth arrest.
The protein may regulate cell growth and proliferation through selective translation of specific mRNA classes.
Multiple processed pseudogenes of this gene are dispersed throughout the genome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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