Thermo Fisher Scientific Stathmin 1 Monoclonal Antibody (SP49)
Stathmin 1 단백질을 인식하는 Rabbit Monoclonal Antibody (SP49). Western blot, IHC, ICC, Flow cytometry에 적합. 인간, 마우스, 랫트 반응성. Protein A/G 정제, PBS 기반 저장용액, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:25–1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:40 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SP49 |
| Immunogen | Synthetic peptide derived from the internal region of human stathmin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2537919 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is tonsil or breast carcinoma tissue.
Target Information
Stathmin 1 belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment.
Stathmin 1 regulates the microtubule filament system by destabilizing microtubules, preventing assembly, and promoting disassembly.
Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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