Thermo Fisher Scientific Thymidylate Synthase Monoclonal Antibody (SP112)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100

Flow Cytometry (Flow)

• Tested Dilution: 1:200

Product Specifications

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 minutes.
Following antigen retrieval, incubate samples with primary antibody for 10 minutes at room temperature.
A suggested positive control is colon carcinoma or tonsil tissue.

Target Information

Thymidylate Synthase (TS) converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis.
TS is a critical target for fluoropyrimidines, an important group of antineoplastic drugs used in the treatment of solid tumors.
Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP, which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate.
Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.