Thermo Fisher Scientific Phospho-Tau (Thr492, Thr175) Polyclonal Antibody

Thermo Fisher Scientific Phospho-Tau (Thr492, Thr175) Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Thr492/175.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker between both structures.
Hyper-phosphorylated Tau is the major component of paired helical filaments (PHF), which form neurofibrillary lesions in Alzheimer’s disease (AD). Phosphorylation impairs Tau’s microtubule binding, leading to destabilization and neuronal degeneration.
Tau phosphorylation is mediated by kinases such as GSK-3β, PKA, cdk5, and casein kinase II. Hyper-phosphorylated Tau is also found in other CNS disorders including Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

Usage Notice

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.