Thermo Fisher Scientific Phospho-Tau (Ser516) Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

This antibody detects endogenous levels of Tau only when phosphorylated at Ser516, which site was historically referenced as Ser199.

Target Information

Tau is a neuronal microtubule-associated protein found predominantly on axons. It promotes tubulin polymerization and stabilizes microtubules. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, functioning as a linker protein between both structures.
Axonal polarity is determined by TAU/MAPT localization within the neuronal cell body. Short isoforms contribute to cytoskeletal plasticity, while longer isoforms aid stabilization.
In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building blocks of neurofibrillary lesions in Alzheimer’s disease (AD) brains. Hyper-phosphorylation impairs Tau’s microtubule-binding ability, leading to destabilization and neuronal degeneration.
Multiple kinases such as GSK-3β, PKA, cdk5, and casein kinase II phosphorylate Tau. Hyper-phosphorylated Tau is also found in other neurodegenerative disorders including Pick’s disease, frontotemporal dementia, corticobasal degeneration, and progressive supranuclear palsy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.