Thermo Fisher Scientific c-Myc Oncoprotein Monoclonal Antibody (MYC275)
c-Myc 단백질을 표적하는 mouse monoclonal antibody로, IHC, ICC, Flow Cytometry 등 다양한 응용에 사용 가능. 인간 시료에 반응하며, 단백질 발현 및 종양 연구에 적합. PBS buffer, 무보존제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MYC275 |
| Immunogen | Recombinant human c-Myc protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The c-Myc protein is a 62 kDa transcription factor encoded by the c-Myc gene on human chromosome 8q24. It is commonly activated in various tumor cells and plays a key role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. Phosphorylation of c-Myc at Thr58/Ser62 by kinases such as GSK3, CDK, ERK2, and JNK is functionally associated with cell proliferation and regulation.
c-Myc is essential for vasculogenesis and angiogenesis in tumor development. The deregulated expression of c-Myc induces apoptosis and is implicated in multiple cancers including lung, breast, and colon carcinomas. Translocation of the c-Myc locus to immunoglobulin loci is characteristic of Burkitt’s lymphoma and other B-cell disorders. Aberrant c-Myc expression occurs in tumors from various tissues such as colorectal, gastric, hepatic, ovarian, pulmonary, and hematopoietic origins.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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