Norgen Animal Tissue RNA Purification Kit

Norgen Animal Tissue RNA Purification Kit

SKU: 25700
For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues.

Features and Benefits

• Extract high quality & quantity total RNA including miRNA
• Isolate from a wide variety of animal tissues
• No phenol step required – isolate all RNA in one fraction
• Compatible with tissues stored in RNAlater® or Trizol®
• Convenient & fast spin column format
• Uses Norgen’s proprietary resin separation matrix

Description

This kit enables isolation of total RNA from a range of animal tissues such as liver, spleen, heart, muscle, and intestine. The tissue is lysed with Buffer RL, followed by Proteinase K treatment to remove fibrous proteins like collagen and contractile proteins. The purified RNA shows excellent RIN values and A260/A280 ratios, suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, and NGS.

The kit purifies all RNA sizes—from large mRNA and lncRNA down to microRNA (miRNA)—in one fraction without phenol. Small RNAs are efficiently bound regardless of GC content.

Supporting Data

Figure 1. High Quality of Isolated RNA with Complete Size Range

Unlike competitor kits, Norgen’s kit isolates all RNA sizes without phenol.
OCR 추출 텍스트:

Competitor        NORGEN
1    2            3    4

Small RNA Species

Figure 2. High Quality RNA Purification from a Diversity of Tissues

Successfully isolates RNA from spleen, kidney, and muscle tissues.
OCR 추출 텍스트:
| Panel | Description |
| ------ | ------------ |
| A | Competitor Muscle |
| B | Norgen Muscle |
| C | Norgen Spleen |
| D | Norgen Kidney |

Figure 3. Total RNA with High Yield of microRNA Species

Norgen kit yields higher microRNA (miR‑21) levels compared to competitors.
OCR 추출 텍스트:

• 상단 그래프: miR‑21
• 하단 그래프: beta‑Actin
• 범례:
  • Norgen — 파란색 선
  • Competitor — 빨간색 선
  • NTC — 노란색 선
• Y축: PCR Base Line Subtracted dRFU
• X축: 0–36 cycles

Figure 4. Compatibility with Tissues Stored in RNAlater®

Norgen kit performs equally well with frozen and RNAlater®‑preserved tissues.
OCR 추출 텍스트:

Y축: RQ or Fluorescence Units
X축: Cycle (0–36)

Kit Specifications

Storage Conditions and Product Stability

• DNase I and Proteinase K: Store at –20°C upon arrival and after reconstitution
• Other solutions: Store tightly sealed at room temperature
• Kit stability: 1 year from shipment date

Kit Components

Documentation

• Protocol (PDF)
• Safety Data Sheet (SDS)
• Flyer

FAQs

Why do I have poor RNA recovery?

Possible causes include incomplete lysis, clogged columns, incorrect elution solution, missing ethanol additions, or low RNA content in tissue.

Why are columns clogged?

May result from insufficient solubilization, excess starting material, high genomic DNA, or low centrifuge temperature.

Why is RNA degraded?

Due to RNase contamination, slow procedure, improper storage, or thawing of frozen tissues before isolation.

Why is RNA not performing well in downstream applications?

Possible salt or ethanol carryover during washing steps.

How to prevent genomic DNA contamination?

Use recommended DNase I digestion and ensure complete passage of DNase mix through the column.

Citations

Recent publications citing this kit include studies in International Journal of Molecular Medicine (2025), Marine Drugs (2025), Annals of Clinical and Translational Neurology (2025), and others.

Related Products

• Leukocyte RNA Purification Kit
• microRNA Purification Kit
• RNase-Free DNase I Kit

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