Thermo Fisher Scientific ApoA1 Monoclonal Antibody (311)
ApoA1 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ELISA 등 다양한 응용에 사용 가능. 인간 및 마우스 시료 반응. Protein A 정제, PBS 버퍼, 5 mg/mL 농도. 냉장 보관(2–8°C), 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution / Concentration |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
| Functional Assay | – |
| Radioimmune Assay (RIA) | Assay-dependent |
| In vitro Assay (IV) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 311 |
| Immunogen | Purified human plasma Apo A-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 2–8°C for up to 6 months; for long-term storage, store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152130 |
Product Specific Information
- By sandwich ELISA, MIA1402 can be used as a detection antibody with Product #710263 or #701239 as a coating antibody to generate a matched pair.
- Detects recombinant human Apo A-1, but not recombinant mouse Apo A-1.
- Suitable for detecting Apo A-1 from serum samples.
- For increased sensitivity in sandwich ELISAs, use a biotinylated detection antibody followed by Streptavidin-HRP.
- By Western blot, detects recombinant human Apo A-1, but may not effectively detect endogenous Apo A-1.
- Previously sold as a Seradyn product.
Target Information
Apolipoprotein A-1 (Apo A-1) is the major protein component of high-density lipoprotein (HDL) in human plasma, with a molecular mass of approximately 24 kDa. It acts as a cofactor for enzymes involved in cholesterol metabolism and is primarily produced in the liver. Plasma concentration ranges from 270–380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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