Thermo Fisher Scientific ApoA1 Monoclonal Antibody (532)
인간 및 마우스 ApoA1에 반응하는 단클론 항체로, WB, IHC, ELISA 등 다양한 응용 가능. 고순도 Protein A 정제, 5 mg/mL 액상 형태. ELISA 매칭 페어 구성 및 고감도 검출에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:100 | |
| ELISA | 1 µg/mL | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Radioimmune Assays (RIA) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 532 |
| Immunogen | Purified human plasma Apo A-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 6 months. For long term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152905 |
Product Specific Information
By sandwich ELISA, MIA1405 can be used as a detection antibody with Product #710263 or #701239 as a coating antibody to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected.
MIA1405 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1405, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1405 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1405 is also recommended for detecting endogenous Apo A-1 by Western blot.
MIA1405 was formerly sold as a Seradyn product.
Target Information
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high-density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270–380 mg/100 mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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