Thermo Fisher Scientific ApoA1 Monoclonal Antibody (513)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

513

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Ion-exchange chromatography

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Wet ice

RRID

AB_11151929

Product Specific Information

By sandwich ELISA, MIA1404 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1404 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1404, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

By Western blot, MIA1404 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1404 is also recommended for detecting endogenous Apo A-1 by Western blot.

MIA1404 was formerly sold as a Seradyn product.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.