Thermo Fisher Scientific ApoA1 Monoclonal Antibody (6001)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

6001

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152877

Product Specific Information

By sandwich ELISA, MIA1401 can be used as a detection antibody with Product # 710263 as the coating antibody, to generate a matched pair. Using this matched pair, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. It is not recommended to use Product # 701239 for coating when MIA1401 is used for detection.MIA1401 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1401, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.

By Western blot, MIA1401 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1401 may not be as successful for detecting endogenous Apo A-1 by Western blot.

MIA1401 was formerly sold as a Seradyn product.

Target Information

Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.