Thermo Fisher Scientific UFD1L Monoclonal Antibody (OTI7C9), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using the carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Ubiquitin-mediated proteolysis involves the transfer of ubiquitin (Ub) to lysine residues on specific cellular proteins, leading to their degradation by the 26S proteasome. Ub-fusions are cleaved by Ub-specific proteases (UBps) or through the Ub-fusion degradation (UFD) pathway.
UFD proteins preferentially cleave Ub-conjugated proteins with an amino acid substitution at the C-terminal glycine residue of Ub. The UFD1 protein, first identified in yeast (S. cerevisiae), has a human homolog known as UFD1L. In vitro, UFD1 attenuates degradation of Ub-fusions containing a proline or valine substitution at Gly76 by promoting selective multiubiquitination.
Mutations in the UFD1 gene are associated with CATCH22 syndrome, which includes cardiac defects, cleft palate, and hypocalcemia, suggesting a role for this pathway in developmental processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.