Thermo Fisher Scientific UFD1L Monoclonal Antibody (OTI5E3), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Ubiquitin-mediated proteolysis involves the transfer of ubiquitin (Ub) to lysine residues on target proteins, leading to their degradation by the 26S proteasome.
The UFD pathway specifically cleaves Ub-conjugated proteins with substitutions at the C-terminal glycine residue.
Human UFD1L, homologous to yeast UFD1, regulates multiubiquitination and degradation of Ub-fusions.
Mutations in UFD1L are associated with CATCH22 syndrome, characterized by cardiac defects, cleft palate, and hypocalcemia, suggesting its role in developmental processes.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.