Thermo Fisher Scientific Cytokeratin, pan Antibody Cocktail
사이토케라틴 전반을 인식하는 Rabbit IgG 칵테일 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. Protein A 정제, 액상 형태로 제공되며, 4°C 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- RBMP49-7249-P1
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Cocktail |
| Type | Antibody |
| Immunogen | Recombinant full-length human KRT76 and KRT77 proteins |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
This antibody is expected to show broad species reactivity.
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes. - Western Blot: Use 1–2 µg/mL for 2 hours at room temperature.
Target Information
Cytokeratin pan is part of a subfamily of intermediate filament proteins characterized by remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides.
Cytokeratins range in molecular weight between 40–68 kDa and have an isoelectric pH between 4.9–7.8.
The various epithelia in the human body express cytokeratins that are characteristic of the epithelium type and related to the degree of maturation or differentiation.
Cytokeratin subtype expression patterns are widely used in distinguishing different epithelial malignancies. These antibodies assist in the differential diagnosis of tumors via immunohistochemistry, and are also valuable tools in cytopathology and flow cytometry.
The composition of cytokeratin pairs varies with epithelial cell type, differentiation stage, growth environment, and disease state.
Keratins are widely used as markers in cancer research and tumor diagnosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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