Thermo Fisher Scientific KLF17/ZNF393 Monoclonal Antibody (PCRP-KLF17-1G2)
KLF17/ZNF393 단백질을 표적하는 Mouse IgG2a 단클론 항체로, WB, ICC/IF, Flow, IP 등 다양한 응용에 적합. 인간 시료 반응성, Protein A/G 정제, 4°C 보관. 세포 성장 및 분화 관련 KLF17 연구에 유용.
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Thermo Fisher Scientific KLF17/ZNF393 Monoclonal Antibody (PCRP-KLF17-1G2)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Immunoprecipitation (IP) | 1–2 µg/100–500 µg lysate |
| Peptide Array (Array) | Assay-dependent |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-KLF17-1G2 |
| Immunogen | Recombinant full-length human KLF17 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody is stable for 24 months.
- Positive Control: HeLa cells
- Cellular Localization: Nucleus
Specificity Comments:
Krüppel-like factors (KLFs) are zinc finger-containing transcription factors involved in cell growth, proliferation, differentiation, and embryogenesis. KLF17 (also known as ZNF393 or zfp393) is a 389 amino acid nuclear protein expressed in testis and ovary, functioning as a germ cell-specific transcription factor in oocyte development and spermatid differentiation. It contains three C2H2-type zinc fingers that bind G/C-rich sites and activate transcription from CACCC-box elements.
Target Information
KLF17 is a transcription repressor that binds to promoters of target genes and inhibits their expression. It negatively regulates epithelial-mesenchymal transition and metastasis in breast cancer by binding to the 5'-CACCC-3' sequence in the promoter of ID1, repressing its expression. It may also function as a germ cell-specific transcription factor involved in spermatid differentiation and oocyte development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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