Thermo Fisher Scientific LIME1 Monoclonal Antibody (OTI3B10), TrueMAB
LIME1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, Lyophilized 형태로 제공. PBS buffer에 trehalose 포함, -20°C 보관. 연구용으로 T세포 신호전달 관련 연구에 적합.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific LIME1 Monoclonal Antibody (OTI3B10), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 30–295 of human LIME1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with a basic cytoplasmic domain expressed by T cells. Upon ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck, Fyn, and their negative regulator Csk.
Csk-mediated phosphorylation of the C-terminal negative-regulatory tyrosine of LIME-associated Lck can increase enzymatic activity compared to the total Lck pool. Thus, LIME functions as a positive regulator of TCR-dependent T cell signaling, though in some contexts, it may mediate inhibitory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.