Thermo Fisher Scientific MERTK Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-499 of human MERTK (NP_0063342) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MERTK,uniProtId:Q12866-1,ncbiNodeId:9606,antigenRange:26-499,antigenLength:999,antigenImageFileName:PA5-120917_MERTK_Q12866-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120917_MERTK_Q12866-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.75 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914489

Product Specific Information

Positive test controls include: HepG2, Mouse spleen. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.

Immunogen sequence: REEAKPYPLF PGPFPGSLQT DHTPLLSLPH ASGYQPALMF SPTQPGRPHT GNVAIPQVTS VESKPLPPLA FKHTVGHIIL SEHKGVKFNC SISVPNIYQD TTISWWKDGK ELLGAHHAIT QFYPDDEVTA IIASFSITSV QRSDNGSYIC KMKINNEEIV SDPIYIEVQG LPHFTKQPES MNVTRNTAFN LTCQAVGPPE PVNIFWVQNS SRVNEQPEKS PSVLTVPGLT EMAVFSCEAH NDKGLTVSKG VQINIKAIPS PPTEVSIRNS TAHSILISWV PGFDGYSPFR NCSIQVKEAD PLSNGSVMIF NTSALPHLYQ IKQLQALANY SIGVSCMNEI GWSAVSPWIL ASTTEGAPSV APLNVTVFLN ESSDNVDIRW MKPPTKQQDG ELVGYRISHV WQSAGISKEL LEEVGQNGSR ARISVQVHNA TCTVRIAAVT RGGVGPFSDP VKIFIPAHGW VDYAPSSTPA PGNA

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.