Thermo Fisher Scientific EEF2K Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 406-725 of human EEF2K (NP_037434.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF2K,uniProtId:O00418-1,ncbiNodeId:9606,antigenRange:406-725,antigenLength:725,antigenImageFileName:PA5-120913_EEF2K_O00418-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120913_EEF2K_O00418-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.86 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914485

Product Specific Information

Positive test controls include: HepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen.

Immunogen sequence: KLDHLHWPVF SDLDNMASRD HDHLDNHRES ENSGDSGYPS EKRGELDDPE PREHGHSYSN RKYESDEDSL GSSGRVCVEK WNLLNSSRLH LPRASAVALE VQRLNALDLE KKIGKSILGK VHLAMVRYHE GGRFCEKGEE WDQESAVFHL EHAANLGELE AIVGLGLMYS QLPHHILADV SLKETEENKT KGFDYLLKAA EAGDRQSMIL VARAFDSGQN LSPDRCQDWL EALHWYNTAL EMTDCDEGGE YDGMQDEPRY MMLAREAEML FTGGYGLEKD PQRSGDLYTQ AAEAAMEAMK GRLANQYYQK AEEAWAQMEE

Target Information

EEF2K is a highly conserved Serine/Threoning kinase in the calmodulin signaling pathway whose activity is increased in cancers including breast cancer. EEF2K (eukaryotic elongation factor 2 kinase) is a threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Up activation, by a variety of upstream kinases including AMPK or TRPM7, EEF2K phosphorylates the elongation factor EEF2 at a single site. Then, EEF2K renders EEF2 unable to bind ribosomes and inactive - in turn, the rate of protein synthesis is reduced.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.