Thermo Fisher Scientific EEF2K Polyclonal Antibody
EEF2K 단백질을 인식하는 Rabbit Polyclonal 항체로, Human 및 Mouse 시료에 반응합니다. Western Blot, ICC/IF, ELISA 등에 사용 가능하며, 고순도 Affinity Chromatography로 정제되었습니다. 액상 형태로 -20°C 보관하며 연구용으로 적합합니다.
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Thermo Fisher Scientific EEF2K Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 406–725 of human EEF2K (NP_037434.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.86 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914485 |
Product Specific Information
Positive test controls include: HepG2, HeLa, LOVO, 22Rv1, 293T, Mouse spleen.
Immunogen sequence:
KLDHLHWPVF SDLDNMASRD HDHLDNHRES ENSGDSGYPS EKRGELDDPE PREHGHSYSN RKYESDEDSL GSSGRVCVEK WNLLNSSRLH LPRASAVALE VQRLNALDLE KKIGKSILGK VHLAMVRYHE GGRFCEKGEE WDQESAVFHL EHAANLGELE AIVGLGLMYS QLPHHILADV SLKETEENKT KGFDYLLKAA EAGDRQSMIL VARAFDSGQN LSPDRCQDWL EALHWYNTAL EMTDCDEGGE YDGMQDEPRY MMLAREAEML FTGGYGLEKD PQRSGDLYTQ AAEAAMEAMK GRLANQYYQK AEEAWAQMEE
Target Information
EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a highly conserved serine/threonine kinase involved in the calmodulin signaling pathway. Its activity is increased in several cancers including breast cancer. EEF2K regulates protein synthesis by controlling peptide chain elongation rate. Upon activation by upstream kinases such as AMPK or TRPM7, EEF2K phosphorylates elongation factor EEF2, rendering it unable to bind ribosomes and reducing protein synthesis rate.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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