Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), eBioscience
Mouse pDC 특이 단백질 Siglec-H를 인식하는 Rat IgG2b 단일클론 항체. Flow cytometry 및 기능 분석에 적합하며, 다양한 형광 접합체 옵션 제공. 0.5 mg/mL 농도, PBS buffer에 sodium azide 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific SIGLEC H Monoclonal Antibody (eBio440c), eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Flow Cytometry (Flow) | 2 µg/test | 14 publications |
| Functional Assay | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio440c |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1107010 |
Available Conjugate Formats
- APC
- Brilliant UV 615
- eFluor 450
- eFluor 660
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Custom conjugation available upon request
Product Specific Information
The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on plasmacytoid dendritic cells (pDCs) or type I IFN-producing cells (IPC) in naive mice.
Mouse IPCs are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viral infections.
Siglec-H is a transmembrane protein of the Ig superfamily, similar to CD33, which binds sialic acid but lacks the cytoplasmic ITIM domain. Instead, it associates with DAP12 to enable signal transduction.
The eBio440c antibody has been shown to inhibit pDC function, reducing IFNα secretion in response to CpG.
Some mouse strains (e.g., SJL) exhibit higher percentages of pDCs compared to C57BL/6.
Applications Tested:
This antibody has been tested by blocking staining of the direct conjugate of eBio440c.
Recommended usage: ≤ 2 µg per test (100 µL final volume).
Cell number can vary from 10⁵ to 10⁸ cells/test.
For flow cytometry, direct conjugates such as Product #11-0333-82 are recommended over purified antibody.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Siglec-H is expressed exclusively on pDCs or type I IFN-producing cells in naive mice.
It is a sialic acid-binding transmembrane protein of the Ig superfamily that lacks an ITIM domain and associates with DAP12 for signal transduction.
The eBio440c antibody can inhibit pDC function and IFNα secretion.
Mouse strain variation affects pDC percentage (e.g., SJL > C57BL/6).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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