Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, Flow Cytometry, IHC, IP 등 다양한 분석에 사용 가능. 0.5 mg/mL 액상 형태로 제공되며 SDS-PAGE 기준 90% 이상 순도. 세포 부착 및 신호전달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 3 |
| Immunohistochemistry (IHC) | - | 45 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | 5 |
| Immunocytochemistry (ICC/IF) | - | 20 |
| Flow Cytometry (Flow) | 0.5 µg/test | 103 |
| Immunoprecipitation (IP) | Assay-Dependent | - |
| ChIP assay (ChIP) | - | 1 |
| Functional Assay | - | 1 |
| Miscellaneous PubMed | - | 3 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Fish, Human, Mouse, Rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467200 |
Available Conjugated Formats
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 421
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation available
Product Specific Information
Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed on endothelial cells, leukocytes, and platelets. CD31 mediates homotypic interactions important for adhesion, cell-cell and cell-matrix interactions, and signal transduction. CD38 has been reported to bind CD31.
Applications Reported:
Suitable for flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It can also be used in in vitro functional assays (Functional Grade purified 390 recommended for functional assays).
Applications Tested:
Tested by flow cytometric analysis of mouse thymocytes and splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell numbers may range from 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is widely expressed in the vascular system, primarily at intercellular junctions. The N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions. CD31 plays key roles in integrin-mediated adhesion, transendothelial migration, angiogenesis, apoptosis, immune regulation, and thrombosis, making it a central molecule in vascular biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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