Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), eBioscience
인간 CD28 항원에 특이적인 단클론 항체로 T세포 활성화 및 공자극 연구에 적합. Flow cytometry, IHC, IP 등 다양한 응용 가능. 고순도(>90%) 및 0.2 µm 여과 처리로 안정성 확보. PBS 완충액에 보관하며 4°C에서 안정적 유지.
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Thermo Fisher Scientific CD28 Monoclonal Antibody (CD28.2), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 2 publications |
| Immunohistochemistry (IHC) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | 56 publications |
| ELISA | - | 1 publication |
| Immunoprecipitation (IP) | Assay-Dependent | 1 publication |
| Functional Assay | Assay-Dependent | 57 publications |
| Inhibition Assays (IA) | - | 1 publication |
| T-Cell Activation (TCA) | - | 4 publications |
| In vitro Assay (IV) | - | 4 publications |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Cynomolgus monkey, Human, Mouse, Non-human primate, Rhesus monkey |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CD28.2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_467193 |
Available Conjugate Formats
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 615 / 737
- Brilliant Violet 480 / 650 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 610-70S / 660-120S / 690
- NovaFluor Red 710 / 725 / 755
- NovaFluor Yellow 660 / 755
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645
- Request custom conjugation available
Product Specific Information
Description:
The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells, and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2), serving as a potent co-stimulator of T cells. Signaling through CD28 enhances IL-2 and IL-2 receptor expression and cytotoxicity of CD3-activated T cells.
Applications Reported:
- Flow cytometric analysis
- Immunoprecipitation (~2–5 µg per million cells)
- Immunohistochemical staining
- Functional assays for T-cell costimulation (use Functional Grade purified CD28.2, Product #16-0289-81)
Applications Tested:
Tested by flow cytometric analysis of normal human peripheral blood cells. Recommended ≤1 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize titer for best results.
Purity and Quality:
- Purity >90% (SDS-PAGE)
- Aggregation <10% (HPLC)
- 0.2 µm post-manufacturing filtration
Target Information
CD28 antigen is a 44 kDa disulfide-linked homodimeric T-cell surface glycoprotein, belonging to the immunoglobulin superfamily. It provides the critical second activation signal to T cells by binding CD80/CD86 on antigen-presenting cells. CD28 prevents T cells from entering anergic states or premature apoptosis. Expressed on most peripheral blood T lymphocytes and thymocytes, with variable levels across maturation stages.
Dysfunction of CD28 is associated with diseases such as mycosis fungoides and Sezary’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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