Thermo Fisher Scientific CD28 Monoclonal Antibody (37.51), eBioscience

Applications and Tested Dilutions

Product Specifications

Additional Formats

• APC
• Biotin
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

Description:
The 37.51 monoclonal antibody reacts with the mouse CD28 molecule, a 45 kDa homodimer expressed by thymocytes, mature T cells, and NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported:
The 37.51 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. It has also been reported in costimulation of T cells in vitro and in vivo.
(Use Functional Grade purified 37.51, Product #16-0281 for functional assays.)

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Note:
Increased incubation times (30–45 minutes) may enhance staining quality.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD28 antigen is a 44 kDa disulfide-linked homodimeric T cell-specific surface glycoprotein of the immunoglobulin superfamily. It is constitutively expressed on most peripheral blood T lymphocytes and acts as a critical costimulatory receptor by binding CD80 and CD86 on antigen-presenting cells. CD28 prevents T cells from entering an anergic state or undergoing premature apoptosis. In mice, CD28 is expressed on all CD4+ and CD8+ T cells in peripheral lymphoid organs and blood, with varying expression levels during thymocyte maturation.
Diseases associated with CD28 dysfunction include mycosis fungoides and Sezary’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.