Thermo Fisher Scientific Nidogen G1 Domain Monoclonal Antibody (JF3)
Nidogen G1 Domain을 인식하는 Rat 모노클로날 항체로, Human 및 Mouse 시료에 반응. ELISA 및 Western Blot에 적합하며 Laminin 결합 억제 기능 보유. 단백질 G 정제, 액상 형태, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Thermo Fisher Scientific Nidogen G1 Domain Monoclonal Antibody (JF3)
Applications and Tested Dilution
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Inhibition Assays (IA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JF3 |
| Immunogen | Recombinant fragment corresponding to the G1Link region of mouse nidogen produced in Fisher rat plus YB2/0 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 2M Tris, tissue culture supernatant, 0.5M acetic acid |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2538490 |
Product Specific Information
MA5-17018 recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin.
MA5-17018 has been successfully used in ELISA and Western blot applications.
The MA5-17018 immunogen is a recombinant fragment corresponding to the G1Link region of mouse nidogen produced in Fisher rat plus YB2/0 cells.
Target Information
Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen).
Entactin is a sulfated glycoprotein widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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