Thermo Fisher Scientific Nidogen G2 Domain Monoclonal Antibody (JF4)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Inhibition Assays (IA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

JF4

Immunogen

Recombinant fragment corresponding to the G2 region of mouse nidogen produced in Fisher rat plus YB2/0 cells

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

2M tris, acetic acid, tissue culture supernatant

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2538489

Product Specific Information

MA5-17017 recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin.

MA5-17017 has been successfully used in ELISA and Western blot applications.

The MA5-17017 immunogen is a recombinant fragment corresponding to the G2 region of mouse nidogen produced in Fisher rat plus YB2/0 cells.

Target Information

Basement membrane components play an important role in cell adhesion, locomotion, differentiation and embryonic compartmentalization. Although the precise composition and structure of basal laminae varies from tissue to tissue, as well as within the same tissue at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans and entactin (or nidogen). Entactin is a sulfated glycoprotein that is widely distributed in basement membranes. It is tightly associated with laminin and also binds to collagen IV. Entactin probably has a role in cell-extracellular matrix interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.