Thermo Fisher Scientific Nidogen G1 Domain Monoclonal Antibody (JF3)
Nidogen G1 Domain을 인식하는 Rat 모노클론 항체(JF3)로, 인간 및 생쥐 시료에 반응합니다. ELISA, Western blot 등 다양한 응용에 적합하며, nidogen-laminin 결합을 억제합니다. 단백질 G 정제, 비결합형 액상 제품으로 연구용으로 사용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: Assay-dependent
ELISA
- Tested Dilution: Assay-dependent
Inhibition Assays (IA)
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JF3 |
| Immunogen | Recombinant fragment corresponding to the G1Link region of mouse nidogen produced in Fisher rat plus YB2/0 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage buffer | 2M tris, 2M acetic acid, tissue culture supernatant |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538491 |
Product Specific Information
MA5-17019 recognizes native and denatured mouse and human nidogen, and blocks binding of nidogen to laminin.
MA5-17019 has been successfully used in ELISA and Western blot applications.
The MA5-17019 immunogen is a recombinant fragment corresponding to the G1Link region of mouse nidogen produced in Fisher rat plus YB2/0 cells.
Target Information
Basement membrane components play an important role in cell adhesion, locomotion, differentiation, and embryonic compartmentalization. Although the precise composition and structure of basal laminae vary from tissue to tissue and at different developmental stages, most mature basal laminae contain type IV collagen, laminins, heparan sulphate proteoglycans, and entactin (nidogen).
Entactin is a sulfated glycoprotein widely distributed in basement membranes, tightly associated with laminin, and also binds to collagen IV. It likely plays a role in cell–extracellular matrix interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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