Thermo Fisher Scientific EGF Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human EGF. Anti-Human EGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EGF (500-P45) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hEGF.

Western Blot:
To detect hEGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P45BT-1MG will be provided as 2 × 500 µg.

Target Information

EGF (Epidermal Growth Factor) binds to the EGF receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to environmental stimuli. EGFR forms homo- or heterodimers with other family members, each initiating distinct signaling cascades via SH2 domain-containing effector proteins.
EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands, acting as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins like GRB2, activating downstream signaling cascades including RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways.
EGF may also play a role in NF-κB activation. Defects in the EGF gene cause hypomagnesemia type 4, and dysregulation is associated with certain cancers.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.