Thermo Fisher Scientific PDGF-AA Polyclonal Antibody, Biotin, PeproTech
PDGF-AA 단백질을 인식하는 Biotin 결합 토끼 폴리클로날 항체입니다. Western blot 및 ELISA에 적합하며, 높은 특이성과 민감도를 제공합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다.
- 카탈로그번호
- 500-P46BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific PDGF-AA Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA (ELISA) | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 28.5 kDa Recombinant Human PDGF-AA |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2930561 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-AA. Anti-Human PDGF-AA-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect hPDGF-AA by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PDGF-AA (500-P46) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hPDGF-AA.
Western Blot:
To detect hPDGF-AA by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant hPDGF-AA is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P46BT-1MG will be provided as 2 × 500 µg.
Target Information
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It is secreted from the alpha-granules of platelets activated during blood coagulation. PDGF exists as three dimeric isoforms (AA, AB, BB), each formed from A and B peptide chains. These isoforms act via two receptor types (alpha and beta) and are potent mitogens for connective tissue cells such as fibroblasts, glial cells, and smooth muscle cells. PDGF also exhibits chemotactic activity for fibroblasts, smooth muscle cells, neutrophils, and mononuclear cells, and plays roles in collagen synthesis, immune modulation, and neuronal survival and regeneration.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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