Thermo Fisher Scientific EGF Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

• AA Sequence of recombinant protein:
  NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

• Preparation:
  Produced from sera of rabbits immunized with highly pure Recombinant Human EGF. Anti-Human EGF-specific antibody was purified by affinity chromatography and then biotinylated.

• Sandwich ELISA:
  To detect hEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
  This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human EGF (500-P45) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hEGF.

• Western Blot:
  To detect hEGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
  Used with compatible secondary reagents, the detection limit for Recombinant hEGF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

• Packaging:
  500-P45BT-1MG will be provided as 2 × 500 µg.

Target Information

Epidermal Growth Factor (EGF) binds to the EGF receptor (EGFR), a 170 kDa protein kinase, initiating diverse cellular signaling pathways. EGFR forms homo- or heterodimers upon activation, recruiting SH2-containing effector proteins and triggering signaling cascades such as RAS-RAF-MEK-ERK, PI3K-AKT, PLCγ-PKC, and STAT pathways.
EGF is expressed in kidney, brain, prostate, and salivary glands, acting as a potent mitogenic factor. Dysregulation of EGF signaling is associated with various cancers and genetic disorders such as hypomagnesemia type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.