Thermo Fisher Scientific CaV3.2 Monoclonal Antibody (N55/10), PE
CaV3.2 단백질을 인식하는 마우스 단클론 항체로, PE 형광 표지되어 세포 분석 및 면역형광 실험에 적합합니다. Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능하며, 인체·마우스·랫트 반응성을 보입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N55/10 |
| Immunogen | Fusion protein amino acids 1019–1293 (II–III loop) of human Cav3.2 |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931852 |
Additional Formats Available:
- Unconjugated (MA5-27656)
- APC (MA5-45395)
- FITC (MA5-45396)
- PerCP (MA5-45397)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45398 was sufficient for detection of Cav3.2 in 10 µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 260 kDa. No cross-reactivity against Cav1.3.
Target Information
This gene encodes a T-type member of the alpha-1 subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio.
The alpha-1 subunit has 24 transmembrane segments and forms the pore through which ions pass into the cell.
Multiple isoforms exist due to alternative splicing. Mutations in this gene have been associated with childhood absence epilepsy (CAE).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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