Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70), FITC
HCN1 단백질을 검출하기 위한 FITC 결합 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 적합. 사람, 생쥐, 랫트 반응성. 단백질 G 정제 및 보존제 무첨가. 4°C 암소 보관 권장.
- 판매단위
- pk
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 | |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| Immunoprecipitation (IP) | Assay-dependent | |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S70 |
| Immunogen | Fusion protein amino acids 778–910 (C terminus) of rat HCN1 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931854 |
Available Formats
- Unconjugated (MA5-27589)
- APC (MA5-45399)
- PE (MA5-45402)
- PerCP (MA5-45401)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45400 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 100 kDa. No cross-reactivity against HCN2.
Target Information
HCN1 (Hyperpolarization-activated cyclic nucleotide-gated channel 1) is part of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. These channels contribute to spontaneous rhythmic activity in both the heart and brain. HCN1 and HCN2 are important for motor learning and neuronal integration by cerebellar Purkinje cells, as well as shaping autonomous neuronal activity and network oscillations. HCN1 is highly expressed in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus, while HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body. Regional variations in HCN channel expression contribute to differences in neuronal pacemaker activity across brain systems.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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